Characterization of Pituitary and Peptide Hormones by Electrophoresis in Starch Gel

Characterization of pituitary and peptide hormones by electrophoresis in starch gel.

In 1957, Poulik (I) described a modification of Smithies’ (2) method of starch gel electrophoresis incorporating a discontinuous buffer system. In his original presentation, Poulik described a peculiar and as yet not fully explained phenomenon of an advancing borate “front” which seemed to be a major factor responsible for the high degree of separation and resolution. In 1960, Ferguson and Wall...

Behavior of the Serum proteins in starch-agar-gel electrophoresis

Caeruloplasmin as demonstrated by starch gel electrophoresis.

Starch gel electrophoresis has demonstrated that graded concentrations of sodium sulphate do not precipitate the serum globulins in any reasonable degree of purity. The use of this form of electrophoresis has shown that caeruloplasmin is associated with a globulin in the Fa(2) position.This technique can be employed as a screening test for patients suspected of suffering from hepatolenticular d...

Distribution of the serum proteins of Syrian hamster as revealed by Starch gel electrophoresis

Starch-gel electrophoresis of rat-serum proteins.

In 1955, Smithies (1) described a procedure for the separation of human serum proteins by horizontal zone electrophoresis in starch gels. The separation of zones was improved by a later modification to a vertical electrophoresis in which the sample was inserted in the gel, free from supporting medium (2). With these methods, it has been demonstrated that many more protein fractions can be separ...